@article{MAKHILLJAVA201413114354,
    title = {Construction and Analysis of Rumen Bacterial Artificial Chromosome Library 
  from a Cashmere Goat Rumen Microflora},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {11},
    pages = {682-687},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.682.687},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.682.687},
    author = {Yu-Lin and},
    keywords = {Rumen microorganism,bacterial artificial chromosome library,Cashmere goat,screening,clone},
    abstract = {A Bacterial Artificial Chromosome Library of rumen microorganism 
  from Shanbei Cashmere goat was
  constructed to study the potential of the ruminant’s ecosystem and the 
  library storage capacity, stability and
  the functionality was also analyzed. The high molecular weight DNA was extracted 
  and purified directly from
  rumen samples in the study by using embedding technology and pulsed field gel 
  electrophoresis approaches.
  After digestion with HindIII, DNA fragments ranging from 50-150 kb was collected 
  and ligated to pCC1BAC
  vector. The ligation mixture was transformed into<I> E. coli</I> TransforMax EPI300. 
  The rumen microorganism BAC
  library contains about 14,400 clones and the average insert size of BAC clones 
  was estimated to be 74.1 kb,
  mostly ranging from 50-90 kb. Based on the data mentioned above, the capacity 
  of the BAC library was about
  1,067 Mb, the frequency of clones without inserts was &lt;2%. The BAC clones 
  show good stability after
  cultured for 100 generations. RFLP analysis showed a great diversity of the 
  insert fragments. Several BAC
  clones with activity of amylase had been screened from the BAC library. Eight 
  clones with amylase activity were
  acquired. It was suggested that the constructed BAC library had good storage 
  capacity, stability and
  functionality and could be used for further research on exploration of enzyme 
  resource and rumen microflora
  of Cashmere goat.}
    }