@article{MAKHILLJAVA201413104343,
    title = {Polymorphism of the <I>Keratin 31</I> Gene in Different Gansu Alpine Fine-Wool Sheep Strains by High Resolution Melting Curve Analysis},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {10},
    pages = {627-632},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.627.632},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.627.632},
    author = {Bihui,Chune,Ruilin,XiaLiang,Jian,Xiaoping,Tingting,Yaojing and},
    keywords = {analysis,wool traits,high resolution melting,Gansu Alpine fine wool sheep,KRT 31},
    abstract = {Keratin Intermediate Filament (KIF) is a key member of the 
  keratin family of proteins. In this study, High-Resolution Melting (HRM) analysis 
  and DNA sequencing were used to detect polymorphisms of the Keratin intermediate-filaments 
  31 (<I>KRT 31</I>) gene in three Gansu Alpine fine wool sheep strains (fine, 
  super fine and dual purpose strain) (n = 448) and to determine impacts of genotype 
  for KRT 31 on expression of wool traits. Results showed that there were a total 
  of two Single Nucleotide Polymorphisms (SNPs) identified as KRT 31 SNP c.166A&gt;G 
  and c.191T&gt;C. The mutation site c.166 (SNP c.166A&gt;G) results in a missense 
  mutation from Asn to Ser. The mutation at site c.191 (SNP c.191T&gt;C) does 
  not alter the amino acid sequence. The &#967;<SUP>2</SUP>-test showed that the 
  genotype distributions in these three strains of the SNP c.166A&gt;G and SNP 
  c.191T&gt;C were not all in agreement with Hardy Weinberg equilibrium. Furthermore, 
  analysis of the impact of <I>KRT 31</I> gene polymorphism on wool traits in 
  three Gansu Alpine fine wool sheep strains indicated that the mean fiber diameter 
  for KRT31c.166A&gt;G genotype AA and AG was smaller than genotype GG (p&lt;0.05) 
  suggesting this mutation could potentially be exploited in gene marker-assisted 
  selection programmes within the wool industry to select for animals with decreased 
  fiber diameter.}
    }