@article{MAKHILLJAVA20141374301,
    title = {Construction of a Chimeric Virus Expressing Mutation Sequences of Classical Swine Fever Virus Yunnan Strain},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {7},
    pages = {470-476},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.470.476},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.470.476},
    author = {Yu-Ai,Yong-Ke,Xiao-Min,Min,Yu-Xin,Ling-Fu,Hong-Bin and},
    keywords = {RNA,CSFV,chimeric virus,construction,genetic stability,infectious molecular clone},
    abstract = {Infectious cDNA clones are a prerequisite for directed genetic 
  manipulation of RNA viruses. To explore the role of mutations in Classical Swine 
  Fever Virus (CSFV) Yunnan strain which caused the atypical clinical signs in 
  pigs, a new pSM derived from CSFV Shiman strain has been constructed and subsequently 
  replaced by the mutation sequences of CSFV YN strain isolated by the laboratory 
  at the positions 1510-1532, 2471-2658, 3152-3176 and 11785-11816 using the targeted 
  recombination strategy to enable rescue of chimeric CSFV. The results showed 
  that chimeric CSFV (vSM-YN) was successfully rescued from PK-15 cells by transfection 
  of the chimeric CSFV RNA transcripts and identified by whole genome sequence 
  analysis, immunofluorescence antibody assay and ELISA detection. Sequencing 
  of the pAC-SM-YN revealed a high genetic stability and the complete genome sequences 
  of rescued viruses vSM-YN after extensive passages in PK-15 cells showed that 
  modifications in pSM were stably maintained. The results indicate that targeted 
  recombination-mediated mutagenesis provides a powerful tool for expediting the 
  construction of novel RNA genomes and should facilitate further study of the 
  pathogenic mechanism of CSFV leading to atypical CSF.}
    }