@article{MAKHILLJAVA20141354260,
    title = {Development and Evaluation of Vaccines in the Mice Using <I>Brucella suis</I> 
  (<I>B. suis</I>) Deletion Mutant of the <I>vjbR</I> Gene},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {5},
    pages = {306-312},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.306.312},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.306.312},
    author = {Chuangfu,Fei,Junbo,Yuanzhi and},
    keywords = {vjbR,vaccines,mice,Brucella suis,(B. suis),gamma interferan},
    abstract = {Brucellosis is an infectious disease that brings great economic burdens for developing countries. The vaccine S2 which is an attenuated <I>Brucella suis</I> (<I>B. suis</I>) strain has been used on a large scale in China. However, the immunity induced by S2 declined relative to those vaccinated with Rev-1 and S19 vaccines. Moreover, the vaccine S2 cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent <I>B. suis</I> vaccine is needed. In this study, a vjbR mutant of <I>B. suis</I> (<I>B. suis</I>&#916;vjbR) was constructed overcome these drawbacks. The <I>B. suis</I>&#916;vjbR strain showed reduced survival capability in RAW264.7 macrophage and mice and induced high protective immunity in mice. In addition, <I>B. suis</I>&#916;vjbR induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of gamma interferon (INF&#947;). Further, the vjbR antigen allowed serological differentiation between natural and vaccinated infection in mice. Therefore, <I>B. suis</I>&#916;vjbR was suggested as a safe and efficacious live vaccine candidate against virulent <I>Brucella suis</I> (<I>B. suis</I>) infection.}
    }