@article{MAKHILLJAVA20141314172,
    title = {MiRNAs Expressed at High Levels in Cardiac Muscle are Associated with Insulin and Calcium Signaling Pathways},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {1},
    pages = {28-36},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.28.36},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.28.36},
    author = {Li,Ya,Jideng,Yingkai,Keren,Liujun and},
    keywords = {miRNA,cardiac muscle,skeletal muscle,deep sequencing,pig},
    abstract = {MicroRNAs (miRNAs) are non-coding RNAs that negatively regulate 
  gene expression. Compelling evidences have demonstrated the important roles 
  of miRNAs in muscle proliferation, differentiation and development processes. 
  Cardiac muscle and skeletal muscle (longissimus dorsi muscle and psoas major 
  muscle) are both striated muscles which perform different functions <I>in vivo</I>. 
  However, little is known about the differences of miRNA expression pattern between 
  these distinct muscles. In this study, researchers applied a deep sequencing 
  approach to identify the miRNA transcriptomes between cardiac and skeletal muscles. 
  Three sequencing libraries were constructed from three 210 days old pigs, a 
  total of 56 M raw data was generated with 95.58% of them were mappable sequences. 
  In total, 321 unique miRNAs were identified in three sequencing libraries of 
  which 109 were differentially expressed. Ranking analysis showed that six out 
  of the ten most highly expressed miRNAs in cardiac muscle were up-regulated 
  compared with longissimus dorsi muscle and psoas major muscle. GO and KEGG pathway 
  analyses for the predicted target genes of the six up-regulated miRNAs showed 
  that the miRNAs highly expressed in CM are mainly involved in insulin and calcium 
  signaling pathways and the constructed miRNA-mRNA interaction networks highlighted 
  the important differences between cardiac and skeletal muscles, all these results 
  may promote the researches on muscle-related diseases in a certain degree.}
    }