@article{MAKHILLJAVA20141344233,
    title = {Construction of Recombinant pPIC9K-AvBD2 and Expression of Chicken AvBD2 
  Mature Peptide in <I>Pichia pastoris</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {4},
    pages = {251-256},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.251.256},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.251.256},
    author = {Kezong,Jian,Xiuheng,Haixia,Xueyan,Kaisong,Lingling and},
    keywords = {AvBD2 mature peptide,P. pastoris,cloning,expression,antibacterial activity},
    abstract = {Avian &#946;-Defensin-2 (AvBD2) is an antimicrobial peptide that plays a significant role in the innate immunity of chickens. In this research, the expression vector pPIC9K-AvBD2 was established and expressed in <I>Pichia pastoris</I>. AvBD2 cDNA was cloned from total RNA of chicken lung by Transcriptase-Polymerase Chain Reaction (RT-PCR). The AvBD2 mature peptide gene was amplified from and cloned into a pPIC9K plasmid. Then the recombinant expression vector pPIC9K-AvBD2 was transformed into <I>P. pastoris</I> GS115 to express the fusion protein. The result showed that a recombinant secretion expression vector pPIC9K-AvBD2 was successfully constructed and a fusion protein about 3.9 kDa chicken AvBD2 was expressed. Densitometric scanning analysis demonstrated that the expressed protein exaccounted for about 63.7% of the total secreted bacterial protein. The expression products had antimicrobial activity to <I>Escherichia coli</I> and <I>Staphylococcus aureus</I> in the Assay of Agar Diffusion Method.}
    }