@article{MAKHILLJAVA20141334193,
    title = {Molecular Characterization and Expression Pattern of a Novel Cadmium/Zinc-Transporting <I>ATPase HMA1</I> Gene},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {3},
    pages = {159-164},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.159.164},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.159.164},
    author = {Wang,Gao,Song and},
    keywords = {Tobacco,gene,cadmium/zinc-transporting ATPase HMA1,expression pattern,established},
    abstract = {Cadmium (Cd) of tobacco is a pollutant that is extremely toxic 
  to the health of humans. Cadmium/zinc-transporting <I>ATPase HMA1</I> gene has 
  been characterized to function in the plant cadmium/zinc-transporting. The complete 
  coding sequence of tobacco cadmium/zinc-transporting <I>ATPase HMA1</I> gene 
  was amplified by RT-PCR. The open reading frame of tobacco cadmium/zinc-transporting 
  <I>ATPase HMA1</I> gene was 2418 bp which encodes a protein of 805 amino acids. 
  BLAST analysis revealed that tobacco cadmium/zinc-transporting ATPase HMA1 protein 
  shares high homology with the cadmium/zinc-transporting ATPase HMA1 of potato 
  (85%), <I>Lycopersicon esculentum</I> (84%), wine grape (71%), sweet orange 
  (71%), soybean (69%) and thale cress (68%). Results also showed that tobacco 
  cadmium/zinc-transporting <I>ATPase HMA1</I> gene has a closer genetic relationship 
  with the cadmium/zinc-transporting <I>ATPase HMA1</I> gene of <I>Lycopersicon 
  esculentum</I>. Prediction of transmembrane helices showed that tobacco cadmium/zinc-transporting 
  ATPase HMA1 might be a transmembrane protein. The expression profile was studied 
  and results indicated that tobacco cadmium/zinc-transporting <I>ATPase HMA1</I> 
  gene was moderately expressed in root, leaf and stem but hardly expressed in 
  flower. These results established the primary foundation of utilizing tobacco 
  cadmium/zinc-transporting <I>ATPase HMA1</I> gene to descrease the cadmium content 
  of tobacco and benefit the health of humans in the future.}
    }