@article{MAKHILLJAVA20141334191,
    title = {Supplementation of β-Mannanase in Diet with Energy Adjustmenton Affect Performance, Intestinal Morphology and Tight Junction Proteins mRNA Expression in Broiler Chickens},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {3},
    pages = {144-151},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.144.151},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.144.151},
    author = {J.J.,A.H.,X.Y.,M.,W.G. and},
    keywords = {B-mannanase,performance,intestinal morphology,intestinal tight junction protein,broiler},
    abstract = {The objective of this study was to assess the effects of commercial 
  β-mannanase enzyme supplementation in low-energy corn-soybean meal diets 
  on performance, intestinal morphology and mRNA expression of intestinal tight 
  junction proteins in 1-21 days broiler chickens. The 1800, 1 day old broiler 
  chicks were divided into 5 treatment groups, 6 pens in each treatment. The study 
  was performed in a randomised complete block design. The 5 treatments were Positive 
  diet Group (PG) with basal energy level, Negative diet Group (NG) with lower 
  energy of 502.08 kJ kg<SUP>-1</SUP> and other groups supplemented with 150, 
  300 and 450 mg kg<SUP>-1</SUP> &#946;-mannanase based on NG. Decrease of energy 
  in NG resulted in loss of the Average Daily Gain (ADG) (p&lt;0.05) and Feed 
  Conversion Ratio (FCR) (p&lt;0.05). Based on the NG, supplementation with &#946;-mannanase 
  in diet significantly improved the Average Daily Gain (ADG) (p&lt;0.05) and 
  Feed Conversion Ratio (FCR) (p&lt;0.05) of birds. ADG of groups with enzyme 
  and lower energy reached to the level of PG. Supplementation with 300 g ton<SUP>-1</SUP> 
  &#946;-mannanase increased the villus height of the small intestine (p&lt;0.05) 
  and 450 g ton<SUP>-1</SUP> &#946;-mannanase significantly decreased the crypt 
  depth (p&lt;0.01). The addition of &#946;-mannanase at 150, 300 and 450 g ton<SUP>-1</SUP> 
  to the diets increased the ratio of crypt depth to villus height of the duodenum 
  and jejunum in birds (p&lt;0.01). The ratio of crypt depth to villus height 
  of the ileum was also increased with 300 and 450 g ton<SUP>-1</SUP> &#946;-mannanase 
  supplementation compared to the low-energy diet group (p&lt;0.01). Chicks fed 
  diet with the &#946;-mannanase showed an increased mRNA expression of ZO-1 in 
  the duodenum compared with the low energy diet (p&lt;0.05). Supplementation 
  with 300 and 450 g ton<SUP>-1</SUP> &#946;-mannanase to the diets enhanced the 
  mRNA expression of Occludin and ZO-1 in the jejunum (p&lt;0.05) and 300 g ton<SUP>-1</SUP> 
  &#946;-mannanase increased enhanced the mRNA expression of Occludin and ZO-1 
  in the ileum (p&lt;0.05). Thus, the addition of &#946;-mannanase to low energy 
  diets improved the performance, gut morphology and mRNA expression of intestinal 
  tight junction proteins.}
    }