@article{MAKHILLJAVA20131213977,
    title = {<I>In vitro</I> Optimization of White Yak (<I>Bos grunniens</I>) Oviduct Epithelial Cells Culture},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {1},
    pages = {99-103},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.99.103},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.99.103},
    author = {Ping,Xian,Xuezhi,Pengjia,Hongbo and},
    keywords = {White yak,oviduct epithelial cell,embryo,culture in vitro,China},
    abstract = {Epithelial cells of the mammalian oviduct play an important 
  role in reproductive and developmental events. Therefore, the objectives of 
  the present study was to establish a method for isolation and culture of White 
  yak (<I>Bos grunniens</I>) oviduct epithelial cells <I>in vitro</I> and to determine 
  the effect of Fetal Calf Serum (FCS), Epidermal Growth Factor (EGF) and Transforming 
  Growth Factor-&#946;<SUB>1</SUB> (TGF-&#946;<SUB>1</SUB>) and their combination 
  on White yak oviduct epithelial cells. The results showed and 100 ng mL<SUP>-1</SUP> 
  of EGF in TCM 199 with 10% FCS could significantly increased cell numbers of 
  yak oviduct epithelial. Alone no addition, EGF (10 ng mL<SUP>-1</SUP>) and TGF-&#946;<SUB>1</SUB> 
  (1 ng mL<SUP>-1</SUP>) had no effect on cell number. However, when included 
  with 10% FCS in medium yak oviduct epithelial cell numbers significantly increased 
  on day 6 of culture. The combination of FCS (10%) and EGF (10 ng mL<SUP>-1</SUP>) 
  is the ideal formula to culture yak oviduct epithelial cells. Co-culture with 
  yak oviduct epithelial cells the developmental rate of blastocyst was 8.9%. 
  The results support the culture environment that oviduct epithelial cell may 
  have direct effects on yak embryo developmental block <I>in vitro</I> production.}
    }