@article{MAKHILLJAVA20131254072,
    title = {Effect of Chinese Medicine Effective Component Prescription of Chickens Immune Organ CD4+, CD8+ After IBDV Infected},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {5},
    pages = {660-663},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.660.663},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.660.663},
    author = {Qiumei,Guisheng,Guangping Gao,Yanying Zhang,Jianzhong Xu and},
    keywords = {Chinese medicine effective component,IBDV,immunomodulator,CD4+,CD8+},
    abstract = {To elucidate the pharmaceutical mechanism about the prevention 
  and treatment effects of Zihuangsan, so as to provide theoretical basis on animal 
  production. The 240, 1 day old chickens were acclimatized for 6 days and then 
  randomly divided into groups A, B, C, D, E and F, 40 birds in each group. The 
  birds in group A, B and C were administered with three dose such as high, middle 
  and low of Chinese Medicine Prescription-Zihuangsan. The chickens in groups 
  D, E and F were given with the same volume of Normal Saline (NS). The birds 
  in groups A, B, C, D and E were infected with IBDV by eye drop, group F as normal 
  control by using NS instead of IBDV and Zihuangsan. The 3 days after IBDV infected, 
  0.5 mL yolk antibody per bird in group D was given intramuscularly. Result show 
  Zihuangsan could significantly reduce the chicken’s 
  mortality and increase the development of positive expression of CD4+, CD8+ 
  T in immune organs such as thymus and intestinal. Zihuangsan could regulate 
  the immune deficiency caused by IBDV. The best dosage of Zihuangsan is middle 
  dose.}
    }