@article{MAKHILLJAVA20131254063,
    title = {Variation in Expression of HSPs and Their Corresponding mRNA Transcripts in the Peripheral Blood Lymphocytes of Beef Cattle by Transportation Durations},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {5},
    pages = {612-617},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.612.617},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.612.617},
    author = {Cong,Li Fan Xu,Zhi Ping Zhang,Chun Yan Zhang,Gao Feng Yang and},
    keywords = {cattle serum,cattle blood plasma,peripheral blood lymphocytes,cattle stress,transportation stress,HSP mRNA,cattle serum,cattle blood plasma,peripheral blood lymphocytes,cattle stress,transportation stress},
    abstract = {The expression of Heat Shock Proteins (HSPs) and corresponding 
  mRNA transcription in Peripheral Blood Lymphocytes (PBL) of transported beef 
  cattle are poorly documented despite potential health implications for consumers. 
  Herein, concentrations of adrenal Cortisol (COR) and Adrenocorticotropic Hormone 
  (ACTH), the expression of HSP70, HSP27, HSP90 and corresponding mRNA transcripts 
  were examined in PBL of transported Xia Nan cattle, the first commercial breed 
  used in China. Six cattle were transported and samples were collected prior 
  to transportation at 1 and 0 h during transportation at 2, 3, 9, 10, 15 and 
  20 h and after transportation at 10, 20 and 30 h. As revealed by radioimmunoassay, 
  COR and ACTH concentrations increased after 2 and 3 h of transportation, likely 
  due to stress. Various HSPs in PBL had unique expression patterns. ELISA results 
  showed that HSP70 expression was initiated at 2 h of transportation and the 
  level was the highest at 2 h of transportation; however, HSP27 and HSP90 were 
  primarily expressed just at 9 h of transportation. HSP70 mRNA transcripts was 
  induced after 3 and 9 h of transportation. Additionally, a significant increase 
  in HSP27 mRNA after 3 h of transportation was observed while no change in HSP90 
  expression was observed during transportation.}
    }