@article{MAKHILLJAVA20131234013,
    title = {Effects of Dietary Vitamin D<SUB>3</SUB> on the Apoptosis, Morphological Structure and Antibacterial Peptide <I>Hepcidin</I> Gene Expression in the Spleen of <I>Monopterus albus</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {3},
    pages = {316-322},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.316.322},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.316.322},
    author = {Ding-Gang,De-Bing,Shan-Shan,Gong-Wei and},
    keywords = {Monopterus albus,vitamin D3,apoptosis,hepcidin gene,morphological structure},
    abstract = {To investigate the effects of dietary Vitamin D<SUB>3</SUB> (VD<SUB>3</SUB>) on the apoptosis, morphological structure and antibacterial peptide <I>hepcidin</I> gene expression in the spleen of <I>Monopterus albus</I> (<I>M. albus</I>), so as to provide theoretical basis for investigating the immunoregulatory functions and mechanisms of VD<SUB>3</SUB>. A total of 540 healthy <I>M. albus</I> (weigh, 21.7&plusmn;2.1 g) were randomly assigned into six groups (3 replicates per group, 30 <I>M. albus</I> per replicate) and fed with dietary VD<SUB>3</SUB> at various concentrations (0 (control), 250, 500, 1000, 2000 and 4000 IU kg<SUP>-1</SUP>). The experiment lasted for 60 days. After feeding experiment, the spleen was collected from 6 <I>M. albus</I> selected randomly from each group for the detection of apoptosis using FCM and antibacterial peptide <I>hepcidin</I> gene using fluorescent quantitative RT-PCR, besides morphological structure was observed using histological method. The Proliferation Index (PI) was significantly increased (p&lt;0.05), the apoptotic rate was significantly reduced (p&lt;0.05), <I>hepcidin</I> gene expression was significantly up-regulated (p&lt;0.05), the percentage of white pulp was increased and more splenic corpuscles were clearly visible in the 1000 IU kg<SUP>-1</SUP> group compared to the control group. There were no significant differences in <I>hepcidin</I> gene expression in the 4000 IU kg<SUP>-1</SUP> group and the number of splenic corpuscle was reduced, gradually degenerating and disappearing. The results demonstrate that appropriate dose dietary provision of VD<SUB>3</SUB> significantly reduced the cell apoptosis rate in the spleen of <I>M. albus</I> whereas promoted the growth of peripheral lymphoid organ and significantly increased <I>hepcidin</I> gene expression, enhancing immunity. However, when the dietary provision of VD<SUB>3</SUB> was excessive or deficient, the apoptosis was increased, cell proliferation was blocked, growth of spleen was inhibited, <I>hepcidin</I> gene expression was down-regulated and immune function was impaired.}
    }