@article{MAKHILLJAVA20131224001,
    title = {Pilot Study on Degradation of Classical Swine Fever Virus Nonstructural 2 Protein in Cells},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {2},
    pages = {234-241},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.234.241},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.234.241},
    author = {Kang-Kang,Qing-Hai,Peng-Bo,He-Lin,Wei,Qi-Zhuang,Wu-Long,Zhi,Cheng-Cheng and},
    keywords = {Classical swine fever virus,NS2 protein,protein degradation,phosphorylation,China},
    abstract = {The Nonstructural 2 (NS2) protein plays an important role 
  in the infection process of Classical Swine Fever Virus (CSFV), it is an autoprotease 
  cleaving the NS2-3 polyprotein for the NS3 protein release. The NS2 protein 
  is an easily biodegradable protein in cells and the biogradation pathways are 
  not still very clear at present. The Swine Umbilical Vein Endothelial Cell (SUVEC) 
  constucted by the lab is a good cell line model for studying the pathogenic 
  mechanism of CSFV. In this study, the degradation of CSFV NS2 were observed 
  by detecting the NS2 protein expression in SUVEC and PK-15 cells. The recombinant 
  plasmid pEGFP-NS2 with complete <I>NS2</I> gene of CSFV was transfected into 
  SUVEC and PK-15 cells, respectively. The NS2 RNA was determined by RT-PCR and 
  expression of NS2 protein in cells were analyzed by fluorescence microscopy 
  and western blot assay after transfected. The degradation of NS2 protein also 
  were observed by fluorescence microscopy and Western-blot assay. The results 
  shown that the NS2 protein is short-lived in cells, the biodegradation process 
  could be inhibited by a proteasomal inhibitor (MG132), hinting the NS2 protein 
  is degraded via proteasome pathway in cells and this degradation process was 
  related to the phosphorylation of NS2 protein. This is a primary study on the 
  degradation of CSFV NS2 protein. The future experiments will address the degradation 
  mechanism of CSFV NS2 and find the phosphorylation amino acids of NS2 protein 
  which maybe relate to protein degadation in cells.}
    }