@article{MAKHILLJAVA20131213962,
    title = {Butyrolactone-I Reversibly Inhibits but does not Improve the Maturation and Subsequent Development of Sheep Oocytes <I>In vitro</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {1},
    pages = {17-23},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.17.23},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.17.23},
    author = {Peishi,Lin,Feige,Yunhai,Hemin,Lieqin,Hong,Junzhong and},
    keywords = {Sheep,oocyte,in vitro maturation,butyrolactone-I,ovine},
    abstract = {Butyrolactone-I (BL-I) which is a selective inhibitor of cyclin-dependent 
  kinases, competitively binds p34<SUP>cdc2</SUP> and inhibits meiotic resumption. 
  However, the function of BL-I in improving the synchronization of nuclear and 
  cytoplasmic maturation of mammalian oocytes <I>in vitro</I> remains unknown. 
  In the present study, the effects of different concentrations and durations 
  of BL-I treatment on the <I>In vitro</I> Maturation (IVM) of sheep oocytes were 
  assessed together with the effects after removal of inhibition for different 
  durations. The developmental competence of ovine oocytes was also determined 
  based on the maturation rate and blastocyst rate of ovine parthenogenetic and 
  <I>in vitro</I> fertilized embryos. The ultrastructure of oocytes treated by 
  BL-I was also observed and analyzed by transmission electron microscopy. The 
  germinal vesicle rate was arrested in 62.9% of oocytes treated by <I>in vitro</I> 
  culture for 24 h with 150 &#956;mol L<SUP>-1</SUP> BL-I. The maturation and 
  blastocyst rates of the treatment groups were 70.83 and 20.33% vs. 63.44 and 
  24.39% in the control but these differences were not significant. The number 
  of blastocysts produced by IVC for 24 h after removal of inhibition was significantly 
  higher than that for the control (71.26&plusmn;32.68% vs. 66.73&plusmn;21.64%, 
  p &gt;0.05). Furthermore, electron micrography analysis also showed some differences 
  with regard to the structure of microvilli, mitochondria and granular cells. 
  The BL-I-induced inhibition of nuclear maturation in ovine oocytes did not appear 
  to play a role in improving their IVM and embryonic development via enhancement 
  of the synchronization of nuclear and cytoplasmic maturation but cytoplasmic 
  maturation was still promoted. In conclusion, BL-I reversibly inhibits IVM of 
  ovine oocytes but does not improve their maturation and subsequent <I>in vitro</I> 
  development.}
    }