@article{MAKHILLJAVA201312214373,
    title = {Polymorphisms of Caprine <I>GDF9</I> Gene and Their Association with Litter Size in Henan Dairy Goat},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {21},
    pages = {1590-1596},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1590.1596},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1590.1596},
    author = {Yuqin,Zhang and},
    keywords = {Goat,growth differentiation factor 9,prolificacy,breeds,genotype},
    abstract = {The Henan Dairy goat is a prolific goat breed in China. The 
  exons 2 of growth differentiation factor 9 (<I>GDF9</I>) gene in three randomly 
  selected does of Henan Dairy goat, Yaoshan goat and Taihang Black goat were 
  amplified and analyzed. The SNPs (T1189C (Val397Ile) and T3615C (Val240Ala) 
  in exon 2) were detected in three goat breeds with different prolificacy, in 
  which T3615C was a new SNP in goats. The product amplified by primers P1 and 
  P2 displayed polymorphisms. For primer P1, three genotypes (GG, GA and AA) were 
  detected in Henan Dairy goats and Yaoshan goats, two genotypes (GG and GA) were 
  found in Taihang Black goat goats and one mutation (1189T&rarr;C) was found 
  by sequencfing in GA genotype. The differences of Least Squares Mean (LSM) for 
  litter size between genotypes GG, GA and AA were significant (p&lt;0.05) in 
  Henan Dairy goat and non-significant (p&gt;0.05) in Taihang Black goat. For 
  primer P2, two genotypes (GG and GA) were detected in three goat breeds and 
  one mutation (3615T&rarr;C) was found by sequencing. The differences of LSM 
  for litter size between genotypes GG and GA were significant (p&lt;0.05) in 
  Henan Dairy goats. These results preliminarily showed that the detected loci 
  of the <I>GDF9</I> gene maybe a major gene that influences prolificacy of Henan 
  Dairy goats.}
    }