@article{MAKHILLJAVA201312204336,
    title = {Optimizing the Method for Cleavage of Ssp DnaB Mini-Intein Mediated Precursor Proteins},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {20},
    pages = {1537-1540},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1537.1540},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1537.1540},
    author = {Lixing,Yu,Ju and},
    keywords = {Optimizing the Method for Cleavage of Ssp DnaB Mini-Intein Mediated Precursor Proteins,cleavage,C-extein,pH,protein},
    abstract = {Inteins are protein splicing elements that excise themselves 
  from precursor protein. It has been widely used to purify recombinant proteins. 
  In the current study, using the Impact-Twin System to express the fusion protein 
  CBD-Ssp DnaB intein-C-extein, researchers investigated the first C-extein residue 
  that may influence the cleavage of Ssp DnaB intein. The results showed that 
  cleavage of the fusion precursor protein was temperature and time dependent 
  and was optimal when pH was 7.0-7.5. Efficiency of cleavage was also, dependent 
  on the first residue of C-extein as well as time and temperature. The data may 
  help in elaborating a facile method for producing other proteins of interest.}
    }