@article{MAKHILLJAVA201312194290,
    title = {A <I>hsp70</I> Gene-Based PCR for Detection of <I>Mycoplasma ovipneumoniae},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {19},
    pages = {1495-1497},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1495.1497},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1495.1497},
    author = {Hua,Cheng,Xiao and},
    keywords = {hsp70 gene,polymerase chain reaction,Mycoplasma ovipneumoniae,detection,China},
    abstract = {<I>Mycoplasma ovipneumoniae</I> is associated with atypical pneumonia in sheep 
  and goats. Traditional detection of <I>M. ovipneumoniae</I> can be complex owing 
  to its slow growth and fastidious nutritional requirements. In present study, 
  researchers developed a PCR Assay Method for specific and sensitive detection 
  of <I>M. ovipneumoniae</I> using primers targeting the heat shock protein 70 
  gene (<I>hsp70</I>). The results demonstrated that the <I>hsp70</I> gene-based 
  PCR is specific by producing an amplicon of 135 bp only with <I>M. ovipneumoniae</I> 
  strains. The detection limit is 4.4 pg which is 10 times more sensitive than 
  the existing 16S rDNA-based PCR. The <I>hsp</I>70 gene-based PCR also demonstrated 
  a higher detection rates for both nasal swabs and lung tissues when comparing 
  with 16S rDNA-based PCR.}
    }