@article{MAKHILLJAVA201312184273,
    title = {Fusion of FedF and SLT-IIe B Subunit is an Candidate Antigen Against Edema 
  Disease of Swine},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {18},
    pages = {1461-1467},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1461.1467},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1461.1467},
    author = {Guoping,Liuqun,Meng-Yuan and},
    keywords = {Ee strain,SLT-IIeB,FedF,fusion expression,immunogenicity},
    abstract = {The DNA sequence encoding the truncated SLT-IIeB and FedF of the <I>Escherichia coli</I> Ee strain which is the major immunogenic fragment for the edema disease in piglets were subcloned and fused to the C terminus of Glutathione S-Transferase (GST) in a pGEX-KG expression vector, to obtain a fusion protein expression plasmid pKSF. Fusion protein was expressed as inclusion body. The anti-serum of GST-SF was able to restrain the toxicity of SLT-IIe to Vero-E6 cells and inhibit the adhesion of F18 fimbriae to the porcine small intestinal brush border cells <I> in vitro</I>. SPF KM mice were vaccinated subcutaneously at 0 and 2 weeks with 25 &#956;g GST-SF, GST-B and GST-F, respectively and were challenged with 5 times the LD<SUB>50</SUB> volume of the Ee strain. The IgG titers against SLT-IIeB or FedF in the GST-SF group were higher and were maintained for a longer time compared to the GST-B and GST-F groups. GST-SF can induce a better immune response and can provide the protection rate of the 70%. These results show the fusion protein GST-SF had stronger immunogenicity and better protective effects against the Ee strain and thus can be used for vaccination against the edema disease of swine.}
    }