@article{MAKHILLJAVA201312164226,
    title = {Molecular Characterization and Expression Pattern of a Novel Flavonoid 3'-Hydroxylase 
  Gene},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {16},
    pages = {1352-1356},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1352.1356},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1352.1356},
    author = {Zhao and},
    keywords = {Flavonoid,tobacco,flavonoid 3`-hydroxylase,expression pattern,flower},
    abstract = {Flavonoids have significant health benefits for animals and humans. Flavonoid 3'-hydroxylase gene have been characterized to be required for flavonoid biosynthesis. In present experiment, the complete mRNA sequence of tobacco flavonoid 3'-hydroxylase gene was amplified using the rapid amplification of cDNA ends methods. The full-length tobacco flavonoid 3'-hydroxylase gene mRNA was 1828 bp containing an 1578 bp open reading frame which encodes a protein of 515 amino acids. Sequence analysis revealed that the flavonoid 3'-hydroxylase genes are conserved in plants. Results also showed that tobacco flavonoid 3'-hydroxylase gene has a closer genetic relationship with the flavonoid 3'-hydroxylase gene of <I>Lycopersicon esculentum</I>. Prediction of transmembrane helices showed that tobacco flavonoid 3'-hydroxylase might be a transmembrane protein. The expression profile was studied and the results indicated that tobacco flavonoid 3'-hydroxylase gene was highly expressed in in leaf and flower. These results established the primary foundation of utilization of tobacco flavonoids in the future.}
    }