@article{MAKHILLJAVA201312164224,
    title = {Phylogenetic Relationships of the <I>18SrRNA </I>Gene Sequence of <I>Histomonas 
  meleagridis</I> from Chickens in East China},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {16},
    pages = {1338-1342},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1338.1342},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1338.1342},
    author = {Jianping,Jinjun,Chanbao,Ping and},
    keywords = {Histomonas meleagridis,18SrRNA,homology,phylogenetic relationships,China},
    abstract = {<I>Histomonas meleagridis</I> (<I>H. meleagridis</I>) is a protozoan parasite that may cause histomoniasis, a disease in poultry of the order Galliformes. The phylogenetic relationships between <I>H. meleagridis</I> in different geographical regions of China are not well understood. By PCR, the partial <I>18SrRNA </I>gene was amplified from 30 liver samples collected from chickens infected with <I>H. meleagridis</I> in East China. The obtained sequences were aligned with other known <I>18SrRNA</I> gene sequences of <I>H. meleagridis </I>strains and two taxonomically related protozoan species. The alignment results showed that the <I>18SrRNA</I> gene sequence of <I>H. meleagridis</I> was highly conserved in East China with nucleotide sequence identity of 98.4-100.0%. Further phylogenetic analysis revealed these sequences were clustered into five groups, suggesting the presence of different genotypes. These results throw a light on molecular diagnosis, epidemiological investigation and population genetics of <I>H. meleagridis</I> in China.}
    }