@article{MAKHILLJAVA201312164220,
    title = {Effect of the Crude Polysaccharides from <I>Poria cocos</I> on mRNA Expression 
  of Adipogenic Factors During 3T3-L1 Preadipocyte Differentiation},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {16},
    pages = {1312-1316},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1312.1316},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1312.1316},
    author = {Hengmin,Xun,Haifeng,Ling,Xi,Hengyong,Yan,Yuhao,Ling and},
    keywords = {Poria cocos,polysaccharides,3T3-L1 preadipocytes,aP2,FAS,Srebf1,GLUT4},
    abstract = {To study the effect of the crude polysaccharides from <I>Poria cocos</I> (PCCP) on mRNA expression of adipogenic factors during 3T3-L1 preadipocyte differentiation. 3T3-L1 preadipocytes were induced to differentiate with MDI (3-isobutyl-1-Methylxanthine, Dexamethasone and Insulin) induction media contained PCCP (50 &#956;g mL<SUP>-1</SUP>) and cultured for 8 days. The extent of the differentiation was assessed by Nile Red staining and GPDH activity. In addition, researchers used real-time quantitative PCR to analyzed the expression of genes related to adipocyte differentiation. The results showed that treatment of differentiating 3T3-L1 preadipocytes with PCCP (50 &#956;g mL<SUP>-1</SUP>) resulted in an increase in the expression of the adipocyte genes such as Fatty Acid System Synthase (FAS), adipocyte fatty acid binding Protein (aP2), insulin-responsive Glucose Transporter (GLUT4) and transcriptional factor genes such as CCAAT/Enhancer Binding Protein &#945; (C/EBP&#945;), peoxisome Proliferator-Activated Receptor gamma (PPAR&#947;) and Sterol Regulatory Element-Binding Protein 1c (SREBP-1c) to some degree. Overall, the data suggested that there was the tendency of increasing the expression of genes related to adipocyte differentiation in PCCP treated cells.}
    }