@article{MAKHILLJAVA201312154214,
    title = {Molecular Characterization and Expression Pattern of a Novel <I>NMNAT1</I> 
  Gene},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {15},
    pages = {1281-1285},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1281.1285},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1281.1285},
    author = {Zhao,Nian,Zhang,Luo,Xia and},
    keywords = {Tobacco,gene,NMNAT1,expression pattern,dinucleotide},
    abstract = {Nicotinamide adenine dinucleotide is important to the health 
  of animals and humans. Nicotinamide Mononucleotide Adenylyltransferase 1 (<I>NMNAT1</I>) 
  gene has been characterized to be required for nicotinamide adenine dinucleotide 
  biosynthesis. In present experiment, the complete mRNA sequence of tobacco <I>NMNAT1</I> 
  gene was amplified using the rapid amplification of cDNA ends methods. The full-length 
  tobacco <I>NMNAT1</I> gene mRNA was 1,445 bp containing a 753 bp open reading 
  frame which encodes a protein of 250 amino acids. BLAST analysis revealed that 
  tobacco NMNAT1 protein shares high homology with the NMNAT1 of <I>Lycopersicon 
  esculentum</I> (87%), wine grape (65%), soybean (65%), cacao (65%) and barrel 
  medic (62%). Results also showed that tobacco <I>NMNAT1</I> gene has a closer 
  genetic relationship with the <I>NMNAT1</I> gene of <I>lycopersicon esculentum</I>. 
  The expression profile was studied and the results indicated that tobacco <I>NMNAT1</I> 
  gene was highly expressed in leaf. These results established the primary foundation 
  of utilizing tobacco nicotinamide adenine dinucleotide or NMNAT1 as drugs for 
  animals and humans in the future.}
    }