@article{MAKHILLJAVA20131213982,
    title = {Cloning and Analysis of Leptin in <I>Culter alburnus</I> in Xingkai Lake 
  and down Regulating its Expression Compared to Cultured Population},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {1},
    pages = {128-134},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.128.134},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.128.134},
    author = {Kun,XiaoTing,RongWei,QiRui,QiRui,GeFeng,XuYang and},
    keywords = {Culter alburnus,Xingkai Lake,Leptin,cloning,expression,wild and cultured population},
    abstract = {Researchers cloned the Leptin of <I>Culter alburnus</I> in 
  Xingkai Lake and analyzed the sequence characteristic of Leptin coding protein 
  and detected the relative expression levels of Leptin RNA in wild and cultured 
  <I>Culter alburnus</I> in Xingkai Lake of different ages. The result provide 
  reference on studying the regulatory effect and molecular mechanism of Leptin 
  on food intake, digestion and catabolism genes and in <I>Culter alburnus</I> 
  in Xingkai Lake. The full length cDNA of <I> Leptin</I> gene was got by RT-PCR, 
  5'-RACE and 3'-RACE. The primer of semi-quantitative RT-PCR and real-time PCR 
  were designed by the acquired cDNA then the gene expression was studied. The 
  full sequence length of Leptin in <I>Culter alburnus</I> in Xingkai Lake was 
  1208 bp, the ORF length is 522 bp which code 173 amino acids. With the increase 
  of age, the expression of mRNA in both wild and cultured <I>Culter alburnus</I> 
  in Xingkai Lake show rising trend, from 3 years old, the expression of Leptin 
  in wild population showed down regulation in contrast to the cultured population.}
    }