@article{MAKHILLJAVA201312154212,
    title = {Molecular Characterization and Expression Pattern of a Novel Nicotinamide Adenine Dinucleotide Transporter Gene},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {15},
    pages = {1271-1275},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1271.1275},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1271.1275},
    author = {Zhao,Nian,Zhang,Luo,Xia and},
    keywords = {Tobacco,nicotinamide adenine dinucleotide transporter 1,expression pattern,protein,wine},
    abstract = {Nicotinamide adenine dinucleotide is a drug which is important 
  to the health of animals and humans. Nicotinamide adenine dinucleotide transporter 
  1 gene have been characterized to be required for nicotinamide adenine dinucleotide 
  bioactivety. In present experiment, the complete mRNA sequence of tobacco nicotinamide 
  adenine dinucleotide transporter 1 gene was amplified using the rapid amplification 
  of cDNA Ends Methods. The full-length tobacco nicotinamide adenine dinucleotide 
  transporter 1 gene <I>mRNA</I> was 1,588 bp containing an 948 bp open reading 
  frame which encodes a protein of 315 amino acids. BLAST analysis revealed that 
  tobacco nicotinamide adenine dinucleotide transporter 1 protein shares high 
  homology with the nicotinamide adenine dinucleotide transporter 1 of <I>Lycopersicon 
  esculentum</I> (93%), wine grape (86%), soybean (81%), chickpea (79%) and foxtail 
  millet (78%). Results also showed that tobacco nicotinamide adenine dinucleotide 
  transporter 1 gene has a closer genetic relationship with the nicotinamide adenine 
  dinucleotide transporter 1 gene of <I>Lycopersicon esculentum</I>. Prediction 
  of transmembrane helices showed that tobacco nicotinamide adenine dinucleotide 
  transporter 1 might be a transmembrane protein. The expression profile was studied 
  and the results indicated that tobacco nicotinamide adenine dinucleotide transporter 
  1 gene was highly expressed in leaf. These results established the primary foundation 
  of using tobacco nicotinamide adenine dinucleotide as drug for animals and humans 
  in the future.}
    }