@article{MAKHILLJAVA20131213979,
    title = {Establishment of a Murine αυβ1 Transgenic CHO-K1 Cell Line 
  and its Susceptibility to Foot-and-Mouth Disease Virus Type Asia l/HN/2006 in China},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {1},
    pages = {108-117},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.108.117},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.108.117},
    author = {H.X.,Y.,Z.D.,Y.,F.,J.J.,W.J.,D.H. and},
    keywords = {Foot-and-mouth disease,foot-and-mouth disease virus,integrin,receptor,cellular},
    abstract = {Field isolates of Foot-and-Mouth Disease Virus (FMDV) were 
  found to use four αυ integrins (αυβ1, αυβ3, 
  αυβ6 and αυβ8) as cellular receptors. Researchers 
  established a stable Chinese Hamster Ovary clone K1 (CHO-K1) cell line expressing 
  the murine αυβ1 heterodimer (designated as CHO-K1-αυβ1) 
  using a highly efficient lentiviral-based gene transfer technology to deliver 
  murine αυ, Internal Ribosome Entry Site (IRES) and β1 genes into 
  cell chromosomes and the inserted genes were then transcribed from a Cytomegalovirus 
  (CMV) promoter. αυβ1 expression was stringently regulated by 
  Doxycycline (Dox) and was found to be stable. CHO-K1-αυβ1 cells 
  were susceptible to FMDV type Asia l/HN/2006. The plaque assay revealed that 
  the virus produced bigger and more plaques in CHO-K1-αυβ1 cells 
  (1.05x10<SUP>4</SUP> PFU mL<SUP>-1</SUP>) than in CHO-K1 cells. When sodium 
  heparin (1 and 2 mg mL<SUP>-1</SUP>) was used as the inhibitor, the number of 
  plaques in CHO-K1 cells were significantly decreased (4.0x10<SUP>3</SUP>-35 
  and 20 PFU mL<SUP>-1</SUP>), supported by time-course of replication and proliferation. 
  The number and size of plaques on CHO-K1-&#945;&#965;&#946;1 cells showed no 
  obvious change, indicating that the &#945;&#965;&#946;1 heterodimer expressed 
  on CHO-K1-&#945;&#965;&#946;1 can be used as an FMDV receptor.}
    }