@article{MAKHILLJAVA201312114143,
    title = {Expression and Study on Immunogenicity of <I>P12A-3A</I> Genes of Type O 
  Foot and Mouth Disease Virus in Recombinant Attenuated Goatpox Virus Strain},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {11},
    pages = {1059-1065},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1059.1065},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1059.1065},
    author = {Wenhui,Jianke,Qiang and},
    keywords = {Promotor P7.5,P12A-3C gene,live vector vaccine,FMDV,immunogenicity},
    abstract = {The <I>P12A</I> gene fragments and <I>3C</I> gene fragments 
  of O/China 99 strains of foot and mouth virus were ligated into bilateral tandem 
  Vaccinia virus promoter P7.5 which could initiate expression of <I>EGFP</I> 
  gene and the expression box p-EGFP-N1-P7.5-P12A-3C was constructed. The expression 
  box was ligated to the linear vector pUC119-TK after KpnI enzyme digestion by 
  blunt end ligation to obtain the recombinant plasmid pUC119-TK-EGFP-P7.5-P12A-3C. 
  The homologous recombination took place in the BHK-21 cell between the recombinant 
  plasmid and the Capripox virus attenuated strain by deleted <I>TK</I> gene. 
  The PCR identification, the Western blot analysis and immunological experiment 
  on mice were carried out. The results showed that the recombinant strain could 
  be subcultured in BHK-21 cell for 10 passages, expressing <I>P12A-3C </I>gene 
  which was 2900 bp by sequence analysis, the Western blot analysis demonstrated 
  that the expressed protein of recombinant plasmid pUC119-TK-EGFP-N1-P7.5-P12A-3C 
  in BHK-21 cells infected GTPV AV41 could be specifically recognized by the hyperimmune 
  serum to type O FMDV. Immunity assay showed that the expression protein had 
  immunogenicity in mice. All the results showed that the Goat Pox virus attenuated 
  strain which could express <I>P12A-3C</I> gene of type O FMDV was obtained.}
    }