@article{MAKHILLJAVA20121173328,
title = {Effects of Different Transport Temperatures on in vitro Development of Queen Oocytes},
journal = {Journal of Animal and Veterinary Advances},
volume = {11},
number = {7},
pages = {995-999},
year = {2012},
issn = {1680-5593},
doi = {javaa.2012.995.999},
url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.995.999},
author = {Alper,Ozen Banu,Asiye Izem,Gul,Cagatay,Sinem Ozlem,Mehmet Can,Guven and},
keywords = {Queen,transport,temperatures,temperatures,in vitro fertilization,Turkey},
abstract = {Today, many species, especially feline are endangered. For
this, domestic cats used as a model for in vitro culture studies. So,
many investigators have problems transporting ovaries to their laboratories.
In this study effects of different transport temperatures on in vitro
maturation of feline oocytes were investigated. Ovaries were collected from
12 ovariectomised queens of 2-3 years old, four of which were at oestrus and
eight at anoestrus. One ovary of each pair was brought to the laboratory in
PBS at 4°C and other one at 37°C. Two main groups as oestrus and anoestrus
were established and each were divided into further 2 subgroups as 4 and 37°C.
Oocytes were collected in TCM-medium and matured for 24 h under 5% CO2
at 38.5°C. Matured oocytes were fertilized with fresh semen at a final concentration
1x106 mL-1 in Sperm-Talp medium under 5% CO2
for 18-24 h. After removing cumulus oophorus cells and zygotes were divided
into groups and in vitro cultured for 72 h in 100 μL SOF medium
under 5% CO2, 5% O2 and 90% N2 gas mixture.
At the 48th h of incubation, the best cleavage was 44.4% (8/18) at 37°C
oestrus group and the lowest was 14% (7/50) in the oestrus group at 4°C.
These rates were 22.72% (15/66) and 28.57% (8/28), respectively for the anoestrus
group. At the 72 h of culture in 37°C oestrus group 7 embryos stayed at
4-8 blastomere stage and 1 embryo reached 16-32 blastomere stage. This result
was significant when compared to the other groups (p<0.001). In 4°C anoestrus
group only 5 embryos have reached 4-8 blastomere and no significant difference
among the results was observed. It is concluded that cat ovaries oestrus are
better transported at 37°C while anoestrus ovaries could be carried at 4°C.}
}