@article{MAKHILLJAVA20121163277,
    title = {Cloning and Sequence Analysis of Donkey Growth Hormone Gene},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {6},
    pages = {731-734},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.731.734},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.731.734},
    author = {Wenjin,Yongmei Su and},
    keywords = {Donkey,growth hormone gene,cloning,sequence analysis,homology,China},
    abstract = {The PCR primers were designed online according to result of gene homology comparison. Donkey <I>GH </I>gene DNA and cDNA sequence were cloned from liver and blood by RT-PCR and PCR, compared with <I>GH</I> gene sequences of different species by bioinformatics. The donkey <I>GH </I>gene sequence was 1928 bp including the 706 bp cDNA sequence with the complete CDS. By comparison for DNA and cDNA sequences, it was found that the <I>GH</I> gene sequence included 5 exons and 4 introns, encoding 216aa including signal protein of 26aa and matured protein of 190aa. Based on the analysis of the similarity of <I>GH</I> genes in different species on the level of cDNA, DNA and the deduced amino acid there was the most homology to the horse. The <I>GH</I> gene of donkey was conservative in the process of evolution and its promotor was not specific TATA box but was CATA box. The mutation of C&rarr;G in 1267 may affect the growth and development of donkeys and horses. All researches made an essential foundation for GH regulation of gene expression, evolution, polymorphism analysis in the future.}
    }