@article{MAKHILLJAVA201211233957,
    title = {Development of Real-Time PCR Methods for the Detection of CD163 and Porcine Reproductive and Respiratory Syndrome Virus <i>N</i> Genes in Marc-145 
  Cell},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {23},
    pages = {4489-4493},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.4489.4493},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.4489.4493},
    author = {Jiming,Ning,Yihong,Angke and},
    keywords = {PRRSV,CD163,SYBR green I,quantitative real-time PCR,China},
    abstract = {The objective of this study was to develop an RNA-dependent 
  real-time reverse-transcriptase PCR (real-time RT-PCR) Method for the detection 
  of relative levels of CD163 and Porcine Reproductive and Respiratory Syndrome 
  Virus (PRRSV) <I>N</I> genes in Marc-145 cells. Primers were designed based 
  on the sequence of highly conservative region of <I>PRRSV N</I>, <I>CD163</I> 
  and <I>&#946;-actin</I> gene as the reference gene. The specificity and sensitivity 
  of real-time RT-PCR Method were determined with the amplification signals Tm 
  peaks in positive controls and the standard curve generated from <I>PRRSV N</I> 
  gene positive plasmid and total RNA dilution end-point standard curve from Marc-145, 
  respectively. The minimum detection levels for <I>PRRSV N</I> gene, <I>CD163</I> 
  and <I>&#946;-actin</I> gene were 10 copies, 0.25 and 0.025 ng total RNA per 
  reaction mixture, respectively. The R<SUP>2</SUP> and efficiency of standard 
  curves were 0.983 and 102.166% for CD163, 1 and 101.453% for PRRSV N and 0.996 
  and 90.969% for <I>&#946;-actin</I> genes. The developed real time RT-PCR Method 
  described in this report is more rapid, specific and sensitive than the conventional 
  RT-PCR for the detection of relative levels of <I>PRRSV N</I> and <I>CD163</I> 
  gene in Marc-145 cell line.}
    }