@article{MAKHILLJAVA201211233947,
    title = {The Effect of <i>Helicobacter pylori</i> on Apoptosis of the GES-1 Transfected pEGFP-N1-UreB Eukaryotic Expression Plasmid <i>in vitro</i>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {23},
    pages = {4435-4440},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.4435.4440},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.4435.4440},
    author = {J.G.,A.X.,L.,J.J.,A.Z. and},
    keywords = {pEGFP-N1-UreB,H. pylori SS1,GES-1,apoptosis,China},
    abstract = {To investigate the effect of <I> Helicobacter pylori</I> on 
  apoptosis of the GES-1 transfected pEGFP-N1-UreB eukaryotic expression plasmid 
  <I>in vitro</I>. The <I>Hp-UreB</I> gene was amplified by PCR and cloned into 
  eukaryotic expression plasmid pEGFP-NI to construct recombinant plasmid pEGFP-NI-UreB 
  and transfected into GES-1 cells and screened by G418. GES-1 cells transfected 
  into plasmid pEGFP-NI-UreB were co-cultured with <I>H. pylori</I> SS1 and its 
  apoptosis was quantified by flow cytometry using Annexin V-APC and 7-AAD. Changes 
  in cell morphology were observed by transmission electron microscope. The recombinant 
  pEGFP-N1-UreB was constructed and stable transfected into GES-1 cells. The apoptotic 
  rates of cells transfected into pEGFP-N1-UreB plasmids were significantly lower 
  than those of control cells (p&lt;0.05).}
    }