@article{MAKHILLJAVA201211233936,
    title = {Cloning and Expression of Chicken AvBD8 Mature Peptide Gene in <i>Lactococcus lactis</i>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {23},
    pages = {4369-4373},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.4369.4373},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.4369.4373},
    author = {Xiuheng,Jian,Kaisong,Liu and},
    keywords = {expression,cloning,AvBD8 mature peptide,Lactococcus lactis,China},
    abstract = {Chicken Beta Defensin-8 (AvBD8) is an antibacterial peptide 
  that plays significant roles in innate immunity. The aim of this study was to 
  investigate the expression of Chicken avian AvBD8 mature peptide gene in <I>Lactococcus 
  lactis</I> (<I>L. lactis</I>) system. In this study, the <I>AvBD8</I> gene was 
  cloned and sequenced from chicken liver by Reverse Transcriptase-Polymerase 
  Chain Reaction (RT-PCR). Then, the AvBD8 mature peptide gene was amplified and 
  cloned into the expression vector pNZ8048. The result showed that the recombinant 
  vector pNZ8048-AvBD8 that verified by colony PCR, double digestion and DNA sequencing 
  was transformed into the competent cell <I>L. lactis</I> NZ9000 by electroporation. 
  The expression of the recombinant protein AvBD8 was confirmed by tricine SDS-PAGE 
  analysis. The present findings suggest that <I>L. lactis</I> could act as a 
  host for the production of avian beta defensins for further study.}
    }