@article{MAKHILLJAVA201211223887,
    title = {Sequence Variation in <I>ROP9</I> Gene among <I>Toxoplasma gondii</I> Strains 
  from Different Hosts and Geographical Locations},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {22},
    pages = {4288-4291},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.4288.4291},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.4288.4291},
    author = {Dong-Hui,Jia,Su-Fang,Zhong-Yuan,Guo-Hua and},
    keywords = {Toxoplasma gondii,toxoplasmosis,Rhoptry Protein 9 (ROP9),sequence variation,phylogenetic analysis},
    abstract = {<I>Toxoplasma gondii</I> infects a wide range of animals and 
  humans causing toxoplasmosis. <I>T. gondii</I> rhoptry protein 9 (TgROP9) is 
  expressed only in tachyzoite stage that might be involved in the early stages 
  of invasion and this soluble protein is constituted with putative B-cell epitope 
  but also can trigger a T cell response and exclusively react with CD4 + T cell 
  clone. In this study, researchers examined sequence variation in <I>ROP9</I> 
  gene among seven <I>T. gondii</I> strains from different hosts and geographical 
  localities by PCR amplification, sequence analysis and phylogenetic reconstruction 
  using Maximum Parsimony (MP). The results showed that the entire genome sequence 
  of the <I>TgROP9</I> gene was 4555 bp in length and no size variation was detected 
  among all the strains. In addition, there were 26 (0-0.7%) variable nucleotide 
  positions among all isolates with 9 variations of these being in the coding 
  region and the other 11 were distributed among the 4 introns. Phylogenetic analyses 
  revealed that the TgROP9 sequences were not a suitable genetic marker to differentiate 
  <I>T. gondii</I> strains of different genotypes from different hosts and geographical 
  locations. This study determined the TgROP9 genomic sequences of seven <I>T. 
  gondii</I> strains and demonstrated the existence of low sequence variation 
  in <I>TgROP9</I> gene, suggesting that <I>T. gondii</I> ROP9 may represent a 
  good vaccine candidate against toxoplamosis.}
    }