@article{MAKHILLJAVA201211223856,
    title = {<I>In vitro</I> Assessment of Gossypol Induced Toxicity in Xinjiang Fine-Wool Sheep Leydig Cell},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {22},
    pages = {4102-4107},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.4102.4107},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.4102.4107},
    author = {Zi-Rong,Tuerxun,Gao-Feng and},
    keywords = {Xingjiang Fine-Wool sheep,Leydig cell,gossypol,toxicity,steroid hormone},
    abstract = {In this research, researchers assessed the gossypol induced 
  toxicity on Xinjiang Fine-Wool sheep Leydig cell to get a better understand 
  of the reproduction toxicity of gossypol on the male ruminant. The Leydig cells 
  with high purity were separated successfully from a testicle of 3 months old 
  Fine-Wool sheep through a method of Percoll Density Gradient Centrifugation. 
  Cytotoxicity assays indicated mitochondrial is sensitive to gossypol induced 
  toxicity. Hoechst staining and DNA-FCM with PI staining revealed gossypol mainly 
  causes cell apoptosis of the Leydig cell. Cell cycle analysis by FCM indicated 
  cell cycle arrest on G0/G1 phase. Leydig cell is the main site of testosterone 
  biosynthesis and testosterone is the critical steroid hormone which determines 
  the initiation and maintenance of spermatogenesis as well as expression of the 
  male phenotype. Thus, cytotoxicity effects of gossypol on the Leydig cell revealed 
  by this research indicated that the reduction of spermatogenesis induced by 
  gossypol in male ruminant at least partially associated with the cytotoxicity 
  effects of gossypol on the Leydig cell.}
    }