@article{MAKHILLJAVA201211213846,
    title = {Clone and Bioinformatics Analysis of Chinese-Belgium Rabbit Metallothionein-1 (<I>MT<SUB>1</SUB></I>), <I>MT<SUB>2</SUB></I> and <I>MT<SUB>3</SUB></I> Genes CDS Region},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {21},
    pages = {4034-4039},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.4034.4039},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.4034.4039},
    author = {Lai,Zhang,Wang,Chen and},
    keywords = {Rabbit,Metallothionein (MT),clone,bioinformatics analysis,China},
    abstract = {Metallothioneins (MTs) play important roles in mental ion metabolism, detoxication of heavy mental, clearance of Reactive Oxygen Species (ROS). In the studies, Coding Sequences (CDS) of <I>MT<SUB>1</SUB></I>, <I>MT<SUB>2</SUB></I> and <I>MT<SUB>3</SUB></I> genes from Chinese-Belgium rabbit were cloned and sequenced. The results showed: CDS length of MT<SUB>1</SUB>, MT<SUB>2</SUB> and MT<SUB>3</SUB> was 186, 186 and 201 bp, respectively, encoded 61, 61 and 66 amino acids, respectively. Amino acid sequence of MTs includes 7 typical Cys-Xaa-Cys motifs and 20 cysteines which form 10 disulfide-bonds. Similarity analysis showed that the similarity of MT<SUB>1</SUB> and MT<SUB>2</SUB> reached to 87.6%; the similarity of MT<SUB>2</SUB> and MT<SUB>3</SUB> was 78.0%; the similarity of MT<SUB>3</SUB> and MT<SUB>1</SUB> was 76.3%. Alignment and cluster analysis of <I>MTs</I> genes from different species suggest MTs are high conserved but different subtype of <I>MTs</I> genes have separated and formed in the early stages of the evolution of species and then they individually evolve.}
    }