@article{MAKHILLJAVA201211213836,
    title = {Characterization of Interferon-α1 of Yak},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {21},
    pages = {3978-3983},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.3978.3983},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.3978.3983},
    author = {ZhiZhong,XuePeng,XiaoBing,YongXiang,Shuang,YaDong,HuaiJie and},
    keywords = {cloning,interferon-a1,Yak (Bos grunniens),antiviral activity assay,Pichia pastoris},
    abstract = {The yak (<I>Bos grunniens</I>) is an important grazing livestock in Western of China. In this study, the full length of <I>yIFN-a1</I> gene was cloned then the recombinant yIFN-a1 was expressed in yeast expression system. <I>In vitro</I> bioactivity of IFN-&#945;1 was determined by inhabition infection BHK cell against Vesicular Stomatitis Virus (VSV). The results showed that cDNA contains an Open Reading Frame (ORF) of 570 base pairs encoding a protein of 189 amino acid (aa) precursor with a signal peptide of 23 aa. The homology of the yIFN-a1 was up to 65.3~94.4% with other species at the amino acid levels. The recombinant yIFN-a1 was recognized in Western blot by mAb against human IFN-&#945;1. The activity unit of the purified recombinant yIFN- a1 protein was 1.16&times;10<SUP>5</SUP> IU mg<SUP>-1</SUP> by against Vesicular Stomatitis Virus (VSV) antiviral assay <I>in vitro</I>. This made a basis for the development of anti-virus medicine of yak in the future.}
    }