@article{MAKHILLJAVA201211213825,
    title = {Cloning and Sequence Analysis of <I>aha1</I> Gene Encoding Major Adhesin Protein from <I>Aeromonas</I> sp. Isolated from Aquaculture Animals with Haemorrhagic Septicemia},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {21},
    pages = {3908-3913},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.3908.3913},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.3908.3913},
    author = {J.N.,W.,L. and},
    keywords = {Aeromonas sp.,identification,aha1 gene,cloning,sequence analysis},
    abstract = {Nine bacterial strains (LA1, LA2, LA4, JA1, CA3, CA4, BA1, BA18 and EA9) were isolated from different aquaculture animals with haemorrhagic septicemia and then they were identified as <I>A. hydrophila</I> (LA4, JA1, CA3 and BA1), <I>A. sobria</I> (LA1 and EA9), <I>A. caviae</I> (BA18) and <I>A. veronii</I> (CA4 and LA2), respectively by morphological and biochemical characterization. All isolates were found to be pathogenic to experimental zebrafish (<I>Danio rerio</I>) by artificial infection test. The outer membrane protein Aha1 is a major adhesin of <I>A. hydrophila</I> and also highly conserved in different serotypes of <I>A. hydrophila</I>. In order to ascertain the conservation of aha1 protein among mesophilic motile aeromonads, full length <I>aha1</I> gene from all isolates was detected, cloned and sequenced. As the results show, the <I>aha1</I> genes were amplified in all strains and the ORF size of the <I>aha1</I> gene from <I>A. hydrophila</I> and other phenotypic species of aeromonas isolates was 1,068 and 1,038 bp, respectively. Four Anhui <I>A. hydrophila </I>isolates and six <I>A. hydrophila</I> reference strains formed a cluster together with 91.4-99.7% nucleotide identity and 91.9-99.7% amino acid identity of the <I>aha1</I> gene. Five Anhui other phenotypic species isolates formed another cluster, they shared 79.5-81.1% nucleotide identity and 79.6-81.6% amino acid identity of the <I> aha1</I> gene compared with <I>A. hydrophila</I> and the major sequence variations were observed between amino acids 85-134, 176- 227, 243-263, 280-295 and 321-336.}
    }