@article{MAKHILLJAVA20121133184,
title = {Influence of Hormones on mRNA Abundance of Enzymes Related Lipid Metabolism in Longissimus Dorsi Muscle in vitro},
journal = {Journal of Animal and Veterinary Advances},
volume = {11},
number = {3},
pages = {386-392},
year = {2012},
issn = {1680-5593},
doi = {javaa.2012.386.392},
url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.386.392},
author = {Gao,Gao,Lu and},
keywords = {muscle explants,mRNA abundance,lipoprotein lipase,Insulin,quantitative real-time PCR,China},
abstract = {The current study was performed to examine the response of
the enzymes related lipid metabolism gene to hormonal stimulation in pig muscle
tissue from finishing pigs. Longissimus dorsi muscle was collected and muscle
tissue explants (approximately 100 mg) were prepared by sterile technique in
finishing barrows (100.55.3 kg BW) of Rongchang. A in vitro system
was established to investigate the regulation of Insulin (INS), Somatotropin
(ST), Epinephrine (Epi) and Glucagon (GCG) on Lipoprotein Lipase (LPL), Acetyl-CoA
Carboxylase (ACC), Fatty Acid Synthase (FAS) and Hormone Sensitive Lipase (HSL)
gene expression to determine whether there was any potential for the manipulation
of the Intramuscular Fat (IMF) deposit in Longissimus dorsi muscle of pig. The
experiment was conducted with an orthogonal design with a L9(34)
and each treatment had three replicates. Results showed that ST significantly
affected HSL and FAS mRNA abundance (p<0.01) and INS influenced LPL and ACC
mRNA abundance (p<0.01), respectively. This results suggested that the combination
of hormones (INS; 1x10–5 M, ST; 5x10–11 M, Epi;
1x10–6 M, GCG; 1x10–7 M, INS;
1x10–7 M, ST; 5x10–9 M, Epi;
1x10–6 M, GCG; 1x10–6 M, INS;
1x10–7 M, ST; 5x10–9 M, Epi;
1x10–6 M, GCG; 1x10–6 M) could
increase IMF content by increasing LPL, ACC and FAS mRNA expression and at the
same time, the combination of hormones (INS; 1x10-7
M, ST; 5x10–10 M, Epi; 1x10–7
M, GCG; 1x10–8 M) had higher IMF content by decreasing
HSL mRNA expression. It could be concluded that the combination of hormones
(INS; 1x10–5, ST; 5x10–9,
Epi; 1x10–7, GCG; 1x10–8)
could improve IMF content in finishing pigs.}
}