@article{MAKHILLJAVA201211203807,
    title = {Prokaryotic Expression of <I>p1</I> Gene of <I>Yersinia ruckeri</I> Isolated 
  from Channel Catfish (<I>Ictalunes punctatus</I>) and Optimization of Expression Conditions},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {20},
    pages = {3800-3805},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.3800.3805},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.3800.3805},
    author = {Kai-Yu,Hai,De-Fang,Jun,Ling-Yuan and},
    keywords = {Yersinia ruckeri,p1 gene,prokaryotic expression,optimization,China},
    abstract = {The <I>p1</I> gene of <I>Yersinia ruckeri</I> (<I>yrp1</I>) 
  which was isolated from channel catfish was amplified by PCR with specific primers 
  and inserted into pMD19-T vector. The positive recombinant plasmid was selected 
  and sequenced. Then, the <I>yrp1</I> gene was subcloned into pET-32a (+) vector 
  and transformed into BL21 (DE3) followed by induction with IPTG and detection 
  with SDS-PAGE. Optimization of the induction conditions were conducted. The 
  results showed that the recombinant protein with a molecular mass of about 72 
  kDa was mostly packaged into inclusion bodies. The optimization of induction 
  process conditions led us to perform the fusion protein induction at 37&deg;C 
  for 4 h with 0.8 mM IPTG.}
    }