@article{MAKHILLJAVA201211183719,
    title = {Comparison of Intradermal Tests, Total Serum IgE Concentrations and Allergen-Specific 
  IgE Using an Arrayed Protein Chip in Atopic Dogs},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {18},
    pages = {3298-3303},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.3298.3303},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.3298.3303},
    author = {Hui-Pi,Pei-Ying and},
    keywords = {Atopic dermatitis,arrayed protein chip,dog,IgE,serum,screening},
    abstract = {Canine atopic dermatitis is a common pruritic skin disease which is most commonly associated with Immunoglobulin (Ig) E immune response to environmental allergens. The aims of this study were to report comparison between total serum IgE concentrations in atopic and non-atopic dogs. Comparison between results of intradermal tests and serum allergen-specific IgE arrayed protein chips was also investigated. Total serum IgE concentrations between 37 atopic dogs (92.5&plusmn;102.3 &#956;g mL<SUP>-1</SUP>) and 34 non-atopic dogs (16.2&plusmn;30.2 &#956;g mL<SUP>-1</SUP>) were significantly different (p&lt;0.001). A positive correlation between total serum IgE concentrations and corresponding modified canine atopic dermatitis extent and severity index scores was found (r = 0.56, p&lt;0.001) in atopic dogs. The cut-off value was 16.8 &#956;g mL<SUP>-1</SUP> with the sensitivity of 91.9% and the specificity of 85.3%. The overall sensitivity of specific IgE serum tests was 24%, specificity was 95% and efficacy was 81%. Total serum IgE concentrations could be a screening test and determine severity of atopic dermatitis in dogs with discrete clinical evaluation. However, arrayed protein chip method might not be suitable for screening functional allergen-specific IgE.}
    }