@article{MAKHILLJAVA201211173709,
    title = {Immunogenicity of Recombinant Attenuated Salmonella Choleraesuis C500 Strain Co-Expressing M and N Protein of Porcine Epidemic Diarrhea Virus (PEDV)},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {17},
    pages = {3234-3241},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.3234.3241},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.3234.3241},
    author = {Qi-Gui,Yu-Peng,Xiao-Yu,Di-Shi,Yu,Li,Lei,Qi,Lin,Zhi-Yong and},
    keywords = {PEDV,membrane protein,nucleocapsid protein,attenuated salmonella,immune response,China},
    abstract = {This study examined the recombinant Attenuated Salmonella 
  Choleraesuis C500 containing <I>M</I> and <I>N</I> gene of PEDV whether can 
  induce a systemic immune responses in mice. After identified by enzyme analysis 
  and nucleotide sequencing test, the constructed recombinant plasmid, designated 
  as pIRES-M-N was transfected into Vero cells. The transient expression protein 
  was detected by indirect Immunofluorescence Assay (IFA) and Western-blotting. 
  Result showed that pIRES-M-N can be expressed correctly in Vero cells. Then, 
  the recombinant plasmid electroporated into <I>S. choleraesuis</I> C500 and 
  the recombinant bacteria was proved to be stabile <I>in vitro</I>. The immunogenicities 
  of this recombinant attenuated salmonella constructs were firstly investigated 
  in a mouse mode. Specific anti-PEDV IgG antibodies and IFN-&#947;, IL-4 of cytokine 
  were detected. The result showed that the recombinant Attenuated Salmonella 
  could induce higher persistence specific hummune responses in mouse by contrast 
  with naked DNA group after 42 days (p&lt;0.05). It also, stimulated a balanced 
  Th1/Th2 immune response. That provided the clinical basic data and theoretical 
  basis for success of the attenuated salmonella vaccine development.}
    }