@article{MAKHILLJAVA201211153620,
    title = {Effect of <i>Gypsum fibrosum</i> and <i>Rhizoma atractylodis</i> on <i>In 
  Vitro</i> Proliferation of Mouse Splenic Lymphocyte},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {15},
    pages = {2687-2691},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.2687.2691},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.2687.2691},
    author = {Yaocheng,Ruiling,Xinguo,Fenghua,Jianqin,Fenghua and},
    keywords = {Qingliang electuary,Shi gao,Bai zhu,in vitro proliferation,mouse splenic lymphocyte,incubation},
    abstract = {Qingliang electuary plays important roles in speeding up heat 
  dissipation, enhancing blood circulation and improving the body’s 
  immune system. The main ingredients of a qingliang electuary are <i>Gypsum fibrosum</i> 
  (GF) and <i>Rhizoma atractylodis</i> (RA). Here, researchers report the effect 
  of these two ingredients on <i>in vitro</i> proliferation of mouse splenic lymphocyte. 
  The extracts of GF and RA along with ConA were tested under high temperatures 
  (37, 39 and 42°C) in combination with different incubation times (1 or 4 
  h) using the MTT (3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di-phenytetrazoliumromide) 
  Method. The results showed that ConA significantly stimulated lymphocyte proliferation 
  at 37°C. Under 39 and 42°C, the stimulation diminished as incubation 
  time prolonged. High dose GF (500 &#956;g mL<sup>-1</sup>) significantly inhibited 
  proliferation under all tested conditions but low dose GF (0.05 &#956;g mL<sup>-1</sup>) 
  significantly increased lymphocyte proliferation at 42°C after 1 h incubation. 
  RA water extract at 500 &#956;g mL<sup>-1</sup> significantly increased lymphocyte 
  proliferation under all tested conditions. The effect of RA oil was weaker compared 
  to RA water extract and not consistent under different incubation conditions.}
    }