@article{MAKHILLJAVA201211153603,
    title = {Interaction Between VirB5 of Brucella Type IV Secretion System (TFSS) and Ferritin Heavy Polypeptide 1 (FTH1) in Murine Macrophage},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {15},
    pages = {2623-2629},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.2623.2629},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.2623.2629},
    author = {Chuangfu,Fei,Yuanzhi,Hui,Jun,Yan,Junbo and},
    keywords = {Yeast two-hybrid,cDNA library,FTH1,Brucella,co-immunoprecipitation},
    abstract = {TFSS is an important virulence factor of Brucella, organized 
  as one operon containing 12 different across the cell wall bacterial proteins 
  among which VirB5 regulates the host phagocytosis of Brucella and the transportation 
  of Brucella in the host cells. This study has constructed cDNA library from 
  <I>Brucella melitensis</I> 16M-infected murine macrophage Raw264.7, identified 
  and confirmed the interaction between Brucella VirB5 and FTH1 of RAW264.7 using 
  yeast two-hybrid and Co-Immunoprecipitation (Co-IP) technologies. Subsequently, 
  the morphological changes and the expression of apoptosis-related genes in Brucella-infected 
  RAW264.7 cells have been investigated with Electron Microscope (EM) and real-time 
  quantitative RT-PCR, respectively. The present study has demonstrated that VirB5 
  and FTH1 play important roles in intracellular parasitism of Brucella and inhibition 
  of FHT1 expression accelerates the apoptosis of macrophage.}
    }