@article{MAKHILLJAVA20121123212,
    title = {Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (<I>Ovis aries</I>) Genes-<I>RHOA, RCHY1</I> and <I>RSU1</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {2},
    pages = {252-259},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.252.259},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.252.259},
    author = {Xia and},
    keywords = {Sheep,RHOA,RCHY1,RSU1,tissue expression,China},
    abstract = {The complete coding sequences of three sheep genes-<I>RHOA</I>,<I> RCHY1</I> and <I>RSU1</I> were amplified using the Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The sheep <I>RHOA</I> gene encodes a protein of 193 amino acids that shares high homology with the ras homolog gene family, member A (RHOA) proteins of fifteen species- cattle (100%), rat (99%), human (100%), mouse (99%), chicken (99%), dog (99%), turkey (99%), sumatran orangutan (99%), poephila guttata (99%), Atlantic salmon (97%), green anole (96%), rainbow trout (96%), African clawed frog (96%), zebrafish (96%) and Western clawed frog (95%). The sheep <I>RCHY1</I> gene encodes a protein of 261 amino acids that shares high homology with the ring finger and CHY zinc finger domain containing 1 (RCHY1) proteins of nine species-cattle (94%), giant panda (92%), pig (91%), human (90%), white-tufted-ear marmoset (90%), chimpanzee (90%), rabbit (89%), mouse (86%) and rat (84%). The sheep <I>RSU1</I> gene encodes a protein of 277 amino acids that shares high homology with the ras suppressor protein 1 (RSU1) proteins of twelve species-cattle (99%), chimpanzee (98%), dog (99%), human (98%), giant panda (98%), horse (98%), rabbit (98%), chicken (96%), mouse (97%), African clawed frog (94%), Western-clawed-frog (94%) and zebrafish (91%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302078, 100302546 and 100302548. The phylogenetic analysis revealed that the sheep <I>RHOA</I>, <I>RCHY1</I> and <I>RSU1</I> genes all have a closer genetic relationship with the <I>RHOA</I>, <I>RCHY1</I> and <I>RSU1</I> genes of cattle. Tissue expression profile analysis demonstrated that sheep <I>RHOA</I>, <I>RCHY1</I> and <I>RSU1</I> genes were all generally but differentially expressed in detected tissues.}
    }