@article{MAKHILLJAVA20121123211,
    title = {Molecular Cloning, Sequence Identification and Tissue Expression Profile of Three Novel Sheep (<I>Ovis aries</I>) Genes-<I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {2},
    pages = {245-251},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.245.251},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.245.251},
    author = {Xia and},
    keywords = {Sheep,ZFAND5,ZGPAT,ZDHHC7,tissue expression,China},
    abstract = {The complete coding sequences of three sheep genes <I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I> were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The sheep <I>ZFAND5</I> gene encodes a protein of 213 amino acids that shares high homology with the Zinc finger, AN1-type Domain 5 (ZFAND5) proteins of eight species; cattle (100%), pig (99%), human (99%), rabbit (99%), mouse (98%), giant panda (98%), gray short-tailed opossum (97%) and Northern white-cheeked gibbon (97%). The sheep <I>ZGPAT</I> gene encodes a protein of 513 amino acids that shares high homology with the Zinc finger CCCH-type with G patch domain-containing protein (ZGPAT) proteins of seven species; cattle (97%), giant panda (84%), rabbit (99%), human (79%), rat (76%), mouse (77%) and chimpanzee (78%). The sheep <I>ZDHHC7</I> gene encodes a protein of 308 amino acids that shares high homology with the Zinc finger, DHHC-type containing 7 (ZDHHC7) proteins of nine species; cattle (99%), dog (93%), pig (93%), human (93%), giant panda (93%), Northern white-cheeked gibbon (92%), mouse (92%), white-tufted-ear marmoset (92%) and rat (92%). Finally, these three novel sheep genes were assigned to GeneIDs; 100302558, 100302028 and 100302557. The phylogenetic analysis revealed that the sheep <I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I> genes all have a closer genetic relationship with the <I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I> genes of cattle. Tissue expression profile analysis was also carried out and results demonstrated that sheep <I>ZFAND5</I>, <I>ZGPAT</I> and <I>ZDHHC7</I> genes were all generally but differentially expressed in detected tissues.}
    }