@article{MAKHILLJAVA201211143573,
    title = {Amplification of Nucleoprotein (<I>NP</I>) Gene from Canine Influenza Virus 
  H3N2 and its Expression in <I>Escherichia coli</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {14},
    pages = {2432-2435},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.2432.2435},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.2432.2435},
    author = {Li,Wang,Guo,Qi,Su,Chen,Zhao,Cao,Zhang,Zhang,Huang,Tan and},
    keywords = {Canine influenza virus,NP gene,cloning,gene expression,protein},
    abstract = {NP is a relatively conservative structure protein also is the main recognition antigen and have good middle cross reactive. To express Canine Influenza Virus (CIV) subtype H3N2 NP protein, the <I>NP</I> gene was amplified by PCR and cloned into vector pMD18-T and then sequence determination. The <I>NP</I> gene was cloned into vector pET-32a (+). The recombinant plasmid pET-NP was transformed into Rosetta II (DE3) and induced with IPTG.}
    }