@article{MAKHILLJAVA201211143567,
    title = {An Improved Reverse Transcription Loop-Mediated Isothermal Amplification Assay 
  for Sensitive and Specific Detection of Infectious Bronchitis Virus},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {14},
    pages = {2398-2402},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.2398.2402},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.2398.2402},
    author = {Yongchang,Hongbin,Daozhong,Chunyi,Jianping and},
    keywords = {Infectious bronchitis virus,loop-mediated isothermal amplification,IBV strains,RT-LAMP},
    abstract = {A highly conserved region of <I>1a </I>gene of Infectious Bronchitis Virus (IBV) was chosen to design the Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) degenerate primers. The developed RT-LAMP assay is a highly sensitive, specific, rapid method to detect IBV in allantoid and tissue. Optimal temperature was around 62&deg;C and duration was 45 min. Sensitivity analysis showed that RT-LAMP assay was 10 fold more sensitive than RT-PCR. Specificity analysis showed that 3 IBV strains omitted by the previous reported RT-LAMP assay could be detected by the improved RT-LAMP assay. In field trials, the improved RT-LAMP assay obtained 98.4% sensitivity in 65 clinical samples while 95.4% of RT-PCR and 93.8% of the previous reported RT-LAMP which indicates it is a powerful tool in the practical application.}
    }