@article{MAKHILLJAVA201211123515,
    title = {Construction of Full-Length Goose Muscle cDNA Library},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {12},
    pages = {2106-2109},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.2106.2109},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.2106.2109},
    author = {Wei,Lu,Haiyang,Yujian,Yingying,Zhe,Jingtao,Tongao and},
    keywords = {China,PCR amplification,SMART technique,cDNA library,Goose},
    abstract = {The muscular tissue of breast was dissected from 8 weeks old Jilin White goose in the present study. The big fragment PCR Method was used to amplify double-strand cDNA based on the SMART techniques for construction of a full-length cDNA library. After digestion with restriction endonuclease Sfi &#124;, a modified vector of pBluescript II SK-plasmid with the adaptors containing Sfi &#124;A and Sfi &#124;B sites was used to recombine with the cDNA products amplified. The recombinants were cloned by transformation into competent <I>Escherichia coli</I> DH2&#945;. A plasmid cDNA library with goose muscle was constructed. The results showed that the titer of the cDNA library was 1.01x10<SUP>6</SUP> pfu mL<SUP>-1</SUP> and the percentage of recombinant clones was 97%. The length of most cDNA inserted was between 0.25 and 1.6 kb identified by gel electrophoresis after cDNA PCR amplification. The unigene ratio was 66.7% and the percentage of complete cDNA sequences was 80% by estimating from the 24 clones sequenced randomly. It is helpful to study muscle development of goose at molecular level in the future.}
    }