@article{MAKHILLJAVA201211123499,
    title = {Transgenic Expression and Antimicrobial Potential of CAMA-Syn in A549 Cells},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {12},
    pages = {2006-2012},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.2006.2012},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.2006.2012},
    author = {Huayan,Junlin,Yulei,Mingming and},
    keywords = {CAMA-syn,lung epithelium,antibacterial assay,antimicrobial peptide,N-terminal,China},
    abstract = {CAMA-syn, a hybrid composed of N-terminal &#945;-helical segment of CA (amino acid 1-8) and N-terminal &#945;-helical segment of MA (amino acid 1-12) is a novel small peptide with the potent antibacterial and synergistic activity without cytotoxicity. In order to test the antibacterial function of CAMA-syn produced specially in lung epitheliuml cells, several vectors containing the synthesized CAMA-syn DNA fragment initiated by CMV and Surfactant Protein C (SP-C) promoter were constructed and transfected into recipient cells. The results showed that CAMA-syn fusion to Green Fluorescent Protein (GFP) or to Hemagglutinin epitope (HA) tag was expressed in both 293 and A549 cells. The antibacterial assays of CAMA-syn were conducted against both Gram positive and negative bacteria including <I>S. abortusovis</I>, <I>P. anatis</I>, <I>S. hyicus</I> and <I>S. suis</I>. The results of colony forming efficiency and cell growth curves proved that the <I>in vitro</I> expressed CAMA-syn could inhibit the growth of bacterial, demonstrating that lung epithelial cell-specific expression of antimicrobial peptide CAMA-syn could have antibacterial activity.}
    }