@article{MAKHILLJAVA201211113476,
    title = {Comparison Between the Effects of Valproic Acid and Trichostatin A on <I>in vitro</I> Development of Sheep Somatic Cell Nuclear Transfer Embryos},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {11},
    pages = {1868-1872},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.1868.1872},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1868.1872},
    author = {Chuangfu,Ren,Wureli,Shengwei,Wei,Zhirui,Wujiafu and},
    keywords = {development-related genes,Valproic acid,SCNT embryo,Trichostatin A,sheep},
    abstract = {The present study was carried out to assess and compare the effects of Valproic Acid (VPA) and Trichostatin A (TSA) on <I>in vitro</I> development of sheep Somatic Cell Nuclear Transfer (SCNT) embryos. The results showed that treatment of cloned sheep embryos with 4 mM VPA or 50 nM TSA for 24 h after activation could significantly improve blastocyst rate compared to the control (30.7 vs. 23.3 vs. 16.7%, respectively p&lt;0.05). VPA treatment resulted in a significant higher blastocyst rate than that of TSA-treated group (p&lt;0.05). Moreover, VPA treatment significantly increased (p&lt;0.05) total cell number per blastocyst compared with the TSA treatment and control groups (78.8&plusmn;9.3 vs. 69.6&plusmn;9.7 vs. 64.1&plusmn;8.6, respectively). Furthermore, VPA treatment increased expression of the development-related genes OCT4 and SOX2 in SCNT blastocysts. These results demonstrate that VPA may be more potent than TSA in supporting developmental competence of cloned embryos.}
    }