@article{MAKHILLJAVA201211113463,
    title = {Interactions of Target Proteins with the <I>virB4</I> Gene from Bovine Embryo Trophoblast Cells Infected with <I>Brucella abortus S</I>creened Through a Yeast Two-Hybrid Assay},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {11},
    pages = {1793-1799},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.1793.1799},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1793.1799},
    author = {Chen,Wang,Zhang,Wang,Li and},
    keywords = {trophoblast,yeast two-hybrid assay,Brucella,virB4,embryo trophoblast cell},
    abstract = {To screen for the interaction of target proteins with the <I>virB4</I> gene in bovine embryo trophoblast cells that were infected with Brucella bacteria. A cell culture for bovine trophoblasts was established. The cDNA library of trophoblast cells infected with the vaccine strain RB51 was constructed and a <I>Saccharomyces cerevisiae</I> expression vector containing the gene <I>virB4</I> was synthesized. Target proteins which interacted with virB4 were screened through a yeast two-hybrid system. The results showed that the cDNA library of bovine trophoblast cells infected with the vaccine RB51 strain was successfully constructed. A recombinant plasmid pGBKT7-virB4 was cloned successfully into the expression vector Y187. Thirteen different types of proteins that interacted with virB4 were screened through a yeast two-hybrid assay.}
    }